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Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation

Hendrickx, Barbara; Junca, Howard; Vosahlova, Jolana; Lindner, Antje; Rüegg, Irene; Bucheli-Witschel, Margarete; Faber, Folkert (Life Sciences & Renewable Energy); Egli, Thomas; Mau, Margit; Schlömann, Michael; Brennerova, Maria; Brenner, Vladimir; Pieper, Dietmar H; Top, Eva M; Dejonghe, Winnie; Bastiaens, Leen; Springael, Dirk

Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allowed …

2006-02-01TechniekArtikel

A novel thermoreversible gelling product made by enzymatic modification of starch

Van Der Maarel, Marc J E C; Capron, Isabelle; Euverink, Gerrit Jan W; Bos, Herman Th; Kaper, Thijs; Binnema, Doede J.; Steeneken, Peter A M

Amylomaltases or D-enzyme (4-α-glucanotransferases; E.C. 2.4.1.25) are carbohydrate-active enzymes that catalyze the transfer of glucan units from one α-glucan to another in a disproportionation reaction. These enzymes are involved in starch metabolism in plants or maltose/glycogen metabolism in many …

2005-10AlgemeenBoekdeel

Transcriptional profiling reveals complex regulation of the monocyte IL-1 beta system by IL-13

Scotton, Chris J; Martinez, Fernando O; Smelt, Maaike J; Sironi, Marina; Locati, Massimo; Mantovani, Alberto; Sozzani, Silvano

IL-4 and IL-13 are prototypic Th2 cytokines that generate an “alternatively activated” phenotype in macrophages. We used high-density oligonucleotide microarrays to investigate the transcriptional profile induced in human monocytes by IL-13. After 8-h stimulation with IL-13, 142 genes were regulated …

2005-01-15AlgemeenArtikel

Fashion Chachacha, Fashion chained and unchained

Jacobs, Dany; Stockert, Andreas

2004AlgemeenAndersoortig materiaal

The application of modelling in the potato production chain

Haverkort, A.J.; Van Haren, R.J.F.

1997AlgemeenConferentiebijdrage

Process for preparing chain-extended starch

Gotlieb, Kornelis Fester; Bruinenberg, Peter Martin; Schotting, Johannes Berdinus; Binnema, Doede Jacob.

Chain-extended starch is prepd. in that starch is polymd. in an aq. soln. at pH 6.0-8.3 by means of glucosyl fluoride in the presence of inorg. phosphate, sucrose phosphorylase, and potato phosphorylase. [on SciFinder(R)]

1994-04-06AlgemeenPatent

The rationale of prevention of thrombosis by enhancing blood levels of single-chain urokinase-type plasminogen activator (scuPA)

Dooijewaard, G; van Boheemen, P A; Binnema, D J; Kluft, C

1992-12-04AlgemeenArtikel

An analysis of the activators of single-chain urokinase-type plasminogen activator (scu-PA) in the dextran sulphate euglobulin fraction of normal plasma and of plasmas deficient in factor XII and prekallikrein

Binnema, Doede J.; Dooijewaard, G.; Turion, P.N.

An analysis was made of the various possible activators of single-chain urokinase-type plasminogen activator (scu-PA) in the dextran sulphate euglobulin fraction (DEF) of human plasma. scu-PA activators were detected in an assay system in which the substrate scu-PA, in physiological concentration (50 …

1991-02-12AlgemeenArtikel

The contact-system dependent plasminogen activator from human plasma

Binnema, D J; Dooijewaard, G; van Iersel, J J; Turion, P N; Kluft, C

Apart from tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA), a third PA appears to occur in human plasma. Its activity is initiated when appropriate triggers of the contact system are added, and the activation depends on the presence of factor XII and prekallikrein …

1990-11-30AlgemeenArtikel

Production of plasminogen activators and inhibitor by serially propagated endothelial cells from adult human blood vessels

van Hinsbergh, V W; Binnema, D; Scheffer, M A; Sprengers, E D; Kooistra, T; Rijken, D C

Endothelial cells were isolated from arteries and veins obtained from elderly people at autopsy and propagated for 37 to 69 population doublings. The cells secreted tissue-type plasminogen activator (t-PA) and PA inhibitor-1, and, after subculturing, urokinase-type PA (u-PA) antigen. The following differences …

1987-07-01AlgemeenArtikel

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