Objective To describe characteristics, risk factors and maternal, obstetric and neonatal outcomes of pregnant women infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Design Multi-centre prospective population-based cohort study. Setting Nationwide study in the Netherlands. Population Pregnant …

The agrifood sector is crucial for achieving global food security and environmental sustainability. In the Netherlands, innovations in food technology and adjacent areas are achieved in attractive projects at Universities of Applied Sciences (UASs) in close interaction with government, industry, other …

Extraintestinal pathogenic Escherichia coli sequence type 131 (ST131), typically fluoroquinolone-resistant (FQ-R) and/or extended-spectrum β-lactamase (ESBL)-producing, has emerged globally. Among clinical isolates, ST131, primarily its H30-R and H30-Rx subclones, accounts for most antimicrobial-resistant …

Purpose: Parentally transmitted germ-line chromothripsis (G-CTH) has been identified in only a few cases. Most of these rearrangements were stably transmitted, in an unbalanced form, from a healthy mother to her child with congenital abnormalities probably caused by de novo copy-number changes of dosage …

Het doel van dit afstudeeronderzoek was het optimaliseren, valideren en implementeren van de dermatofyten Polymerase Chain Reaction (PCR) voor het real-time PCR apparaat LightCycler 480. De detectie van de dermatofyten vindt plaats door middel van de amplificiatiecurve en de determinatie gebeurt aan …

Het poliovirus bestaat uit drie serotypen. Deze serotypen kunnen poliomyelitis veroorzaken bij mensen en primaten. Dit kan resulteren in spierverlammingen met lichaamsmisvorming of dood tot gevolg. Om poliomyelitis te voorkomen zijn er in de loop der jaren twee soorten vaccins ontworpen. Het geïnactiveerde …

In het St. Antonius ziekenhuis(AZN) is er een onderzoek gestart om de klonale verwantschap tussen twee longtumoren, afkomstig van één patiënt, te bepalen met Next Generation Sequencen (NGS). Er wordt hierbij in 50 genen gezocht naar mutaties. Als de twee longtumoren overeenkomende mutaties hebben in …

Purpose With current diagnostic methods, the majority of patients with symptomatic colorectal anastomotic leakage (CAL) is identified approximately 1 week after operation. The aim of this study is to determine whether real-time polymerase chain reaction (RT-PCR) for detection of Escherichia coli and …

tmoA and related genes encode the alpha-subunit of the hydroxylase component of the major group (subgroup 1 of subfamily 2) of bacterial multicomponent mono-oxygenase enzyme complexes involved in aerobic benzene, toluene, ethylbenzene and xylene (BTEX) degradation. A PCR-denaturing gradient gel electrophoresis …

Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allowed …